Scientists Revive 46,000-Year-Old Roundworms From Siberian Permafrost

The nematodes had survived in a state of slowed metabolism called cryptobiosis, according to a new paper

Permafrost melts down a cliff side above a river
Permafrost melts into the Kolyma River outside of Zyryanka, Russia, in 2019. The worms in the new study seem to have survived buried deep in the permafrost for tens of thousands of years. Michael Robinson Chavez / The Washington Post via Getty Images

From 46,000-year-old Siberian permafrost, a team of researchers has collected and revived prehistoric roundworms, according to a new study published Thursday in the journal PLOS Genetics.

The parasitic microorganisms seem to have remained alive by entering a state called cryptobiosis, in which they reduce their metabolism to extremely low levels to withstand extreme conditions. Essentially, the worms were frozen in time.

The researchers calculated the age of the roundworms, or nematodes, by radiocarbon dating plant material also found in the permafrost. They determined the organisms are members of a previously unknown species, which they’ve dubbed Panagrolaimus kolymaensis, after the Kolyma River near the site the worms were found.

“We can say that they are alive, because they move, they eat bacteria on the culture plates, and they reproduce,” Philipp Schiffer, a co-author of the study and evolutionary biologist at the University of Cologne in Germany, tells the Washington Post’s Carolyn Y. Johnson.

“To have a complex and multicellular organism that can shut down and go into this state of suspended animation—for all extents and purposes appear dead... that’s mind-boggling,” says Thomas Boothby, a molecular biologist at the University of Wyoming who didn’t contribute to the research, to the Wall Street Journal’s Dominique Mosbergen.

When organisms enter cryptobiosis, their metabolic, reproductive, developmental and repair processes grind to a halt, according to the University of Hawaii at Manoa. Past studies have found examples of organisms surviving for long periods of time in this state. For example, a Bacillus bacterial spore lived in amber for between 25 million and 40 million years, and a Lotus seed germinated after spending 1,000 to 1,500 years in an ancient lake, per the paper. Previously, the longest a nematode had been recorded to survive in cryptobiosis was just 39 years.

The discovery of the long-frozen nematodes was first outlined in a 2018 study that estimated the worms, found in a fossil arctic gopher burrow, were 42,000 years old, according to Scientific American’s Meghan Bartels. The new work pushes their age back an additional 4,000 years and claims they’re part of a previously unknown species, which typically lives for only one to two months, per the Washington Post.

“The age over which it survived is one of the shocking things,” Gregory Copenhaver, a biologist at the University of North Carolina at Chapel Hill who was not an author of the study but edited it for PLOS Genetics, tells the Washington Post.

But Byron Adams, a biologist at Brigham Young University who wasn’t involved in the work, tells Scientific American that he’s not as sure about the parasites’ age—the study only confirms the age of the surrounding plant material, not the worms themselves. “The authors haven’t done the work to show that the animals they have recovered are not simply surface contaminants,” he tells the publication.

The researchers learned more about the worms by spawning more than 100 generations of them in the lab. Like other species of Panagrolaimus, the newly discovered one reproduces asexually through parthenogenesis, and it has three full sets of chromosomes, instead of the usual two.

While the original, revived worms are no longer alive, some of their offspring are still thriving. These nematodes seem to use a sugar called trehalose to protect against dehydration—and when frozen, this substance has been shown to protect cells, according to the Wall Street Journal.

One day, studying organisms that use cryptobiosis could reveal ways to preserve human cells, per the publication.

“Perhaps you can develop molecules that achieve the same things,” Craig Marshall, a biochemist at the University of Otago in New Zealand who did not participate in the research, tells the Wall Street Journal.

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